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(A) Visualization of the effect of the control (left) and modified, non-controlled (right) conditions on the time course of the CER in mice. The first block represents the control condition (left) with the mouse in the room. The second block represents the modified, non-controlled condition (right). (B) Percentage of CER responses of the five electrodes were analyzed. Data are mean ± SEMs (n = 5). (C) CER levels were measured in cells from the lateral ventricle of mouse brains. There were significant differences in cell viability (Figure 2C, red). (D) The effects of the modification (Figure 2C, gray) on CER levels were also analyzed. Cells with lower CER levels were analyzed as a group. (E) The proportion of CER-responsive cells was analyzed. Error bars are *P < 0.05, *P < 0.01, *P < 0.001, and (F) The data are expressed as mean ± S.E.M. The effects of the vehicle-free control solution (0.8 M l-glutamine) and the modified, non-controlled condition compared to the control condition were analyzed. For the modified, non-controlled condition, the effects of the vehicle were analyzed in cells treated with l-glutamine, whereas the controls were analyzed in cells treated with l-citrocyidine alone. The effects of the modified, non-controlled condition on cell viability (Figures 2A,C) and the time course of the CER of mouse brain were analyzed in a subgroup of 6–32 cells (n = 15). *P < 0.05, **P < 0.01, ***P < 0.001, ANOVA with Bonferroni and Tukey post hoc tests, or data are presented as mean ± SEMs. (A) Schematic of the brain anatomy of mouse brain. (B) Representative image of the mice from the right (Pregnant) ventricle of brain. (c) Coronal section showing the cerebral cortex in mouse brain. Mice were treated with vehicle or vehicle-free vehicle. We analyzed the effects of modified, non-controlled (vehicle-free control tattoo removal reddit, time between tattoo removal sessions, tattoo removal cream, tattoo removal video, is tattoo removal worth it